Skip to main content
Home

Dedicated to discovery

  • Company
    • About us
    • Careers
    • Events
  • Support
    • Laboratory Planning
  • Contact
  • EN
  • DE
  • ES
  • FR
  • IT
  • PT
Home
  • Products
    • PURELAB®
      • PURELAB® Dispenser
      • PURELAB® Flex
      • PURELAB® Quest
      • PURELAB® Pharma Compliance
      • PURELAB® Chorus 1 Complete
      • PURELAB® Chorus 1
      • PURELAB® Chorus 2
      • PURELAB® Chorus 3
      • Purelab Chorus 2+ (RO/DI/UV) / (RO/EDI/UV)
    • CENTRA®
      • CENTRA® R60/120
      • CENTRA® R200
      • CENTRA® RDS
    • MEDICA®
      • MEDICA® 150
      • MEDICA® 7/15
      • MEDICA® BIOX 2024
      • MEDICA® Pro-R & Pro-RE
      • MEDICA® R200
      • MEDICA® EDI 15/30
      • MEDICA® Pro-LPS
      • MEDICA® Pro EDI 60/120
      • MEDICA® BIOX
      • Hubgrade
    • BIOPURE®
      • BIOPURE® 300/600
    • PURENERGY 30
    • ELGA Full Product Range
  • Applications
    • Atomic Absorption Spectroscopy
    • Cell and Tissue Culture
    • Clinical Biochemistry
    • Electrochemistry
    • Gas Chromatography
    • General lab water requirement
    • High Performance Liquid Chromatography (HPLC)
    • Immunochemistry
    • Liquid Chromatography
      • High Performance Liquid Chromatography
    • Mass Spectrometry
    • Microbiology
    • Molecular Biology
    • Spectrophotometry
  • Technologies
    • Activated Carbon
    • Electrodeionization (EDI)
    • Filtration
    • Ion Exchange
    • PureSure
    • Reverse Osmosis
    • Ultraviolet
  • Impurities In Water
    • Dissolved Gases
    • Inorganic Compounds
    • Microorganisms & Bacteria
    • Organic Compounds
    • Particulates
  • Knowledge
    • BROSCHÜREN
    • Blog
    • Case Studies
    • Ultrapure water
    • Guides and White Papers
  • Where to buy
  • Contact us
Home
  • Contact us
  • Products
    • PURELAB®
      • PURELAB® Dispenser
      • PURELAB® Flex
      • PURELAB® Quest
      • PURELAB® Pharma Compliance
      • PURELAB® Chorus 1 Complete
      • PURELAB® Chorus 1
      • PURELAB® Chorus 2
      • PURELAB® Chorus 3
      • Purelab Chorus 2+ (RO/DI/UV) / (RO/EDI/UV)
    • CENTRA®
      • CENTRA® R60/120
      • CENTRA® R200
      • CENTRA® RDS
    • MEDICA®
      • MEDICA® 150
      • MEDICA® 7/15
      • MEDICA® BIOX 2024
      • MEDICA® Pro-R & Pro-RE
      • MEDICA® R200
      • MEDICA® EDI 15/30
      • MEDICA® Pro-LPS
      • MEDICA® Pro EDI 60/120
      • MEDICA® BIOX
      • Hubgrade
    • BIOPURE®
      • BIOPURE® 300/600
    • PURENERGY 30
    • ELGA Full Product Range
  • Applications
    • Atomic Absorption Spectroscopy
    • Cell and Tissue Culture
    • Clinical Biochemistry
    • Electrochemistry
    • Gas Chromatography
    • General lab water requirement
    • High Performance Liquid Chromatography (HPLC)
    • Immunochemistry
    • Liquid Chromatography
      • High Performance Liquid Chromatography
    • Mass Spectrometry
    • Microbiology
    • Molecular Biology
    • Spectrophotometry
  • Technologies
    • Activated Carbon
    • Electrodeionization (EDI)
    • Filtration
    • Ion Exchange
    • PureSure
    • Reverse Osmosis
    • Ultraviolet
  • Impurities In Water
    • Dissolved Gases
    • Inorganic Compounds
    • Microorganisms & Bacteria
    • Organic Compounds
    • Particulates
  • Knowledge
    • BROSCHÜREN
    • Blog
    • Case Studies
    • Ultrapure water
    • Guides and White Papers
  • Where to buy
  • Contact us
  • Company
    • About us
    • Careers
    • Events
  • Support
    • Laboratory Planning
  • Contact
  • EN
  • DE
  • ES
  • FR
  • IT
  • PT
  • Privacy policy
  • Terms & Conditions
  • Global Legal Compliance
  • Patents
  • Trademarks
  • Impressum
  • LC-MS/MS and Ultrapure Water - An Essential Partnership for Clinical Research
Analytical Chemistry
Clinical & Pharma

LC-MS/MS and Ultrapure Water - An Essential Partnership for Clinical Research

3 May 2021
- by Dr Paul Whitehead

Science Drops

Looking through the scientific literature there are a great variety of applications involving analysis by liquid chromatography - tandem mass spectrometry (LC-MS/MS) covering the analyses of virtually all types of complex organic mixtures.  

It is one of the most useful and valuable analytical techniques available. One thing that these applications have in common is that they all use ultrapure water (UPW). Modern LC-MS would not be half as powerful a technique if UPW were not used to minimise impurities in the preparation of samples, standards, blanks and mobile phases. These blogs are to remind the reader of the range of these LC-MS/MS and UPW applications in clinical research taking some recent examples of work reported using UPW from PURELAB Chorus 1 units.

LC-MS/MS is widely used to study the roles and fates of medicines and toxins in body fluids, plasma and hair. Roman Bellouard and colleagues1 reported on the simultaneous determination of B-lactam antibiotics in human plasma and cerebrospinal fluid. Sample clean-up included protein precipitation with acetonitrile followed by evaporation and reconstitution of the residue with mobile phase solvents. LC separation was on a C18 column with gradient elution with water and acetonitrile. Detection was by tandem MS. Total run time was only 8 min. It was used to perform therapeutic drug monitoring on over 2000 patients.

Cyanate is a toxin adversely affecting patients with chronic kidney disease and also Renkecz et al.2  developed a method to quantify cyanate in rat plasma and applied it to toxicokinetic bioanalysis. The cyanate was derivatized using aged solutions of anthranilic acid before determination by LC-MS/MS. There was scope to adapt the method to provide information on cyanate concentrations in patients with chronic kidney disease for therapeutic support.

Hair analysis is a very useful technique for monitoring exposure to drugs used in long-term therapy and in drug abuse, with applications in both forensic work and clinical toxicology. Jihyun Kim and his colleagues3  have developed and validated a rapid LC-MS/MS method to determine 8 antiepileptic drugs – 6 barbiturates and phenyltoin and topiramate - in segmented hair. Methanol was found to be the best extractant. The limits of detection were 0.01ppm for topiramate and 0.5ppm for the others. Hair from drug users showed levels of about 10ppm of phenolbarbital and 0.5ppm topiramate.https://s33626.pcdn.co/wp-content/uploads/2016/02/Triple_quadrupole_schematic.jpeg.optimal.jpeg

 

 

 

 

References:

  1. Simultaneous determination of eight β-lactam antibiotics in human plasma and cerebrospinal fluid by liquid chromatography coupled to tandem mass spectrometry Ronan Bellouard, Guillaume Deslandes, Clément Morival, Julien Li, David Boutoille, Pascale Jolliet, Éric Dailly, Matthieu Grégoire Journal of Pharmaceutical and Biomedical Analysis, Volume 178, 30 January 2020, 112904
  2. Development and Validation of an LC–MS-MS Method for the Quantification of Cyanate in Rat Plasma and Its Application to Toxicokinetic Bioanalysis Tibor Renkecz, Sirma Scopchanova, Gábor Hirka, Ilona Pasics Szakonyiné Journal of Analytical Toxicology, bkaa163, https://doi.org/10.1093/jat/bkaa163 12 October 2020
  3. Simultaneous determination of barbiturates, phenytoin and topiramate in hair by LC-MS/MS and application to real samples Jihyun Kim, Junghyun Kim, Hyesun Yum, Moonhee Jang, Jongsook Rhee, Sangki Lee, Sang Beom Han Journal of Pharmacological and Toxicological Methods Vol 106, November–December 2020, 106931

Dr Paul Whitehead 

After a BA in Chemistry at Oxford University, Paul focused his career on industrial applications of chemistry. He was awarded a PhD at Imperial College, London for developing a microwave-induced-plasma detector for gas chromatography. He spent the first half of his career managing the analytical support team at the Johnson Matthey Research/Technology Centre,specialising in the determination of precious metals and characterising applications such as car-exhaust catalysts and fuel cells. Subsequently, as Laboratory Manager in R&D for ELGA LabWater, he has been involved in introducing and developing the latest water purification technologies. He now acts as a consultant for ELGA.

  • Sales Enquiry
  • Get a Quote
  • Technical Support
  • Find an Approved Partner

Sales Enquiry

At ELGA LabWater, we have exciting offers and news about our products and services that we hope you’d like to hear about. We will use your information to predict what you might be interested in. We will treat your data with respect and you can find the details in our Privacy Policy.

ELGA LabWater works with a network of Approved Partners. In order to answer your questions or enquiries, we may pass your contact details to an Approved Partner, who may contact you directly. 

I'd like to receive updates from ELGA LabWater & ELGA Approved Partners based on my details.

Get a Quote

At ELGA LabWater, we have exciting offers and news about our products and services that we hope you’d like to hear about. We will use your information to predict what you might be interested in. We will treat your data with respect and you can find the details in our Privacy Policy.

ELGA LabWater works with a network of Approved Partners. In order to answer your questions or enquiries, we may pass your contact details to an Approved Partner, who may contact you directly. 

I'd like to receive updates from ELGA LabWater & ELGA Approved Partners based on my details.

Technical Support

At ELGA LabWater, we have exciting offers and news about our products and services that we hope you’d like to hear about. We will use your information to predict what you might be interested in. We will treat your data with respect and you can find the details in our Privacy Policy.

ELGA LabWater works with a network of Approved Partners. In order to answer your questions or enquiries, we may pass your contact details to an Approved Partner, who may contact you directly. 

Call us

Can't find what you are looking for?

Support Number
+44 (0)20 3567 7300
United Kingdom Sales
+44 (0)1628 879 704
United States of America Sales
+1 877-315-3542
France Sales
+33 1 40 83 65 00
China Sales
+86 400-616-8882

 

ELGA LabWater US Headquarters

ELGA LabWater North America
5 Earl Ct Suite 100
Woodridge, IL 60517
USA

Tel: 630-343-5251

Case Studies

  • Abbott Diagnostics
  • DASA Medical Diagnostics
  • NeoDIN Medical Institute
  • North Staffordshire NHS Trust
  • Olsberg Vocational College

Resources

  • Learn About Ultrapure Water
  • Guides and White Papers
  • Purification Technologies
  • Applications
  • Impurities In Water
  • Latest Blog
  • Water Purity - Different Types of Pure Water
  • What is Clinical Laboratory Reagent Water (CLSI)?
  • What is Total Organic Carbon (TOC)?

© VWS (UK) Ltd. trading as ELGA LabWater. 2025 - All rights reserved.
ELGA is the global laboratory water brand name of Veolia.

  • Privacy policy
  • Terms & Conditions
  • Global Legal Compliance
  • Patents
  • Trademarks
  • Impressum
  • Language
    • Deutsch
    • English
    • Español
    • Français
    • Italiano
    • Português
    • 日本語
    • 中文
  • Other Veolia Sites
    • Veolia
    • Veolia Foundation
    • Veolia Water Technologies
Elga Veolia
TOP

© 2017 ELGA Veolia